Salivary Cortisol & Hormone Rhythm Testing: Uncover the Hidden Drivers of Mood, Sleep & Energy

Your mood, your sleep, your energy — they’re all shaped by the invisible dance of hormones and stress chemistry inside your body. At BalanSoul, we use salivary cortisol & hormone testing to capture that dance in real time. By measuring your stress hormones at four key points across the day, we can see whether your cortisol is rising like rocket fuel in the morning and settling down by night — or whether the rhythm has gone flat, flipped, or chaotic.

When cortisol is too high or too low at the wrong times, it can disrupt sleep, sap energy, and amplify anxiety or depression. And when hormones drift off balance, the neurotransmitters that shape mood and resilience — serotonin, dopamine, melatonin — shift with them.

This page will walk you through why these patterns matter for mental health, and how testing can reveal the hidden drivers of anxiety, depression, fatigue, or insomnia. More importantly, it shows how BalanSoul translates lab numbers into a roadmap for rebalancing your biochemistry — helping you feel calmer, sleep better, and regain the energy and stability you’ve been missing.

Why saliva (not blood) for steroid hormones
  • Saliva measures the “free” hormone fraction— the part that’s bioavailable and active in your tissues. Blood tests often include protein-bound forms that don’t act on cells.
  • Steroid hormones are fat-soluble.Saliva reflects this lipophilic state better than serum, giving a clearer picture of how stress hormones behave in real life.
  • Stress-free collection.Timed saliva samples capture your natural rhythm at home, without the needle-induced stress spike that can distort results.
Why BalanSoul needs to know your cortisol rhythm

At BalanSoul, we see cortisol as the fuel that runs your system — like petrol in a car or rocket fuel in an engine. In the morning, it should surge like a shot of natural caffeine, sparking energy and focus. By night, it should taper so your body can rest and melatonin can rise.

When this rhythm drifts out of sync, mental health often follows. Many people with anxiety, depression, or burnout show cortisol curves that are flattened, blunted, or flipped. Instead of fuelling energy at the right time, cortisol works against them — leaving them drained in the morning, restless in the evening, or trapped in a “wired but tired” state.

This is where BalanSoul’s approach is unique. We don’t just measure cortisol as “high” or “low” — we map its behaviour across four points in the day. That map shows whether your cortisol is in step with your biology or out of rhythm. With that insight, we design a personalised plan to restore its natural rise-and-fall pattern — so you wake with energy, unwind at night, and feel steady in between.

👉 At BalanSoul, restoring cortisol rhythms is often the turning point that helps clients regain energy, mood stability, and restorative sleep — in ways medication alone cannot achieve.

Why BalanSoul needs to know your hormone balance

At BalanSoul, we see hormones as the orchestra conductors of your biochemistry. When they’re in tune, everything plays together smoothly — mood, sleep, energy, and focus. But when they drift off-key, the entire symphony of neurotransmitters shifts with them.

In women, estrogen — particularly estradiol (E2) — is a powerful example. Around menopause, E2 declines. Estradiol helps convert tryptophan into serotonin, the neurotransmitter that lifts mood and steadies sleep. As E2 falls, serotonin production weakens, and with it comes irritability, low mood, and insomnia. And because serotonin is the raw material for melatonin, lower estrogen often means less melatonin at night.

At BalanSoul, we see this pattern weekly in saliva profiles of women in perimenopause and menopause: low estradiol, lower melatonin, and disrupted sleep feeding into mood changes.

In men, testosterone plays a parallel role. During andropause, testosterone gradually declines, and with it, the drive, resilience, and focus it once supported. Neurotransmitters shift too — leaving many men feeling flat, unmotivated, or depressed.

👉 Hormone testing matters at BalanSoul because it reveals these hidden biochemical shifts. With that roadmap, we can guide hormones back into harmony, so neurotransmitters — and your life — can follow suit.

Hormones and neurotransmitters: how they interact
  • Estradiol and serotonin:Estradiol (E2) up-regulates tryptophan hydroxylase (enzyme for serotonin synthesis), modulates serotonin transporters (SERT), and influences 5-HT receptors. This helps explain perimenopausal mood and sleep changes as E2 declines.
  • DHEA and mood:Research suggests DHEA may counter cortisol’s effects, interact with neurotransmission and immune signalling, and show antidepressant-like effects in studies.

👉 At BalanSoul, this is why we pair cortisol mapping with sex hormone analysis — together they give the clearest picture of mood and resilience.

The Adrenocortex Stress Profiles (choose the panel that fits you)

 

Adrenocortex Stress Profile — Basic (1001)

  • Markers:Cortisol (x4), DHEA-S
  • Price:$118
  • Best for:Budget-conscious clients wanting a simple stress rhythm snapshot.
  • Why it matters:Provides a clear view of daily cortisol behaviour and resilience (via the DHEA:cortisol ratio), but does not capture sex hormone shifts that often drive mood and sleep.

Adrenocortex Stress Profile — Female Basic (1010)

  • Markers:Cortisol (x4), DHEA-S, Estradiol (E2), Estrone (E1), Estriol (E3), Progesterone, Testosterone, ratios (P4:E2, estrogen quotient)
  • Price:$263
  • Best for:Women with PMS/PMDD, perimenopause, menopause, mood swings, sleep issues, or low libido.
  • Why it matters:Shows the combined effect of stress and female hormones — explaining patterns like low serotonin and melatonin during menopause that affect mood, sleep, and energy.

Adrenocortex Stress Profile — Male Basic (1011)

    • Markers:Cortisol (x4), DHEA-S, Testosterone, Progesterone, Estrone (E1), Estradiol (E2), Estriol (E3), ratios
    • Price:$193
    • Best for:Men with low energy, stress intolerance, low motivation, reduced libido, or recovery issues.
    • Why it matters:Connects cortisol with testosterone/estrogen balance — showing whether stress is “stealing” from anabolic pathways that fuel mood, drive, and resilience.

 

 

Comparison table
Responsive Comparison Table
Panel Price Best for Cortisol x4 DHEA S Female Hormones Male Hormones Ratios Add ons
1001 Basic $118 Budget friendly overview all genders DHEA Cortisol Melatonin Sleep profile
1010 Female $263 Mood sleep cycle perimenopause postmenopause Melatonin Sleep profile
1011 Male $193 Energy mood libido stress testosterone balance Melatonin Sleep profile

Optional add-on: Melatonin (sleep timing)

  • Salivary melatonincan be added if sleep disturbance is your primary concern.
  • Identifies whether your brain is making melatonin at the right time.
  • Cost: $36as a melatonin add-on to other profiles.

What each profile can reveal:

  • Cortisol patterning (4-point):
  • High at night → difficulty falling asleep / “tired-but-wired”
  • Low in the morning → unrefreshed, low drive
  • Flattened curve → burnout / fatigue phenotype
    • DHEA-S & DHEA:cortisol ratio:Reflects stress adaptation, resilience, and catabolic/anabolic balance. Low ratios are linked with depression and post-surgical stress; high ratios with panic disorders.
    • Female panel (1010):Shows luteal adequacy, estrogen quotient, and P4:E2 balance — relevant to PMS/PMDD, perimenopause mood swings, and sleep disturbance.
    • Male panel (1011):Highlights androgen status, aromatisation to estrogens (E1/E2/E3), and implications for mood, energy, and body composition.

Your Testing Journey: From Ordering to Results

STEP 1

STEP 1

Your Test Kit Arrives
Your kit is mailed after payment and includes all forms and instructions.
STEP 2

STEP 2

Collect Your Sample
At home (urine, saliva, or blood spot) follow kit instructions. Or at a collection centre bring your kit and request form.
Test instructions will confirm which collection method applies
STEP 3

STEP 3

Return Your Sample
Use the reply-paid envelope or courier satchel provided. Some centres or couriers send directly for you.
All kits include return packaging and instructions nothing extra to arrange.
STEP 4

STEP 4

Results Ready
Once your sample reaches the lab, testing begins. We’ll contact you to book your results consultation.
Some test results may be available within a week, while others (especially complex profiles) may take up to 3 weeks.

Adrenocortex Stress Profile — Basic (1001)

  • Cortisol (x4: morning, midday, afternoon, bedtime)
  • DHEA-S
  • DHEA:Cortisol ratio

Adrenocortex Stress Profile — Female Basic (1010)

  • Cortisol (x4)
  • DHEA-S
  • Estradiol (E2), Estrone (E1), Estriol (E3)
  • Progesterone
  • Testosterone
  • Ratios: P4:E2, Estrogen Quotient

Adrenocortex Stress Profile — Male Basic (1011)

  • Cortisol (x4)
  • DHEA-S
  • Testosterone
  • Progesterone
  • Estrone (E1), Estradiol (E2), Estriol (E3)
  • Ratios: P4:E2, Estrogen Quotient
Your kit includes everything needed for simple at-home saliva collection:
    • 4 labelled saliva collection tubes (for morning, noon, evening, and bedtime)
    • Step-by-step instructions
    • Requisition form
    • Prepaid return satchel

1️⃣ Prepare

  • Collect samples at the specified times: immediately on waking, midday, late afternoon, and before bed.
  • Avoid food, caffeine, smoking, brushing teeth, or chewing gum for 30 minutes before collection.

2️⃣ Collect

  • Fill each tube with saliva to the marked line.
  • Label clearly with date and time of collection.

3️⃣ Store

  • Keep tubes refrigerated or frozen until ready to return.

4️⃣ Return

  • Place tubes in the provided biohazard bag.
  • Complete the requisition form.
  • Send back using the prepaid satchel (preferably early in the week to avoid weekend delays).

💡 Important: Follow the timing instructions closely — accurate cortisol mapping depends on samples being taken at the correct times of day.

 

 Optional add-on: Melatonin

  • Can be added to any panel if sleep disturbance is a main concern.
  • Identifies whether melatonin is being produced at the right times.
  • Price: $36
How to choose
    • On a budget?Start with the Basic (1001). It’s affordable and maps your cortisol rhythm.
    • Want the full picture?The Female (1010) or Male (1011) panels are strongly recommended, as they capture both stress hormones and sex hormone balance.

    👉 At BalanSoul, we recommend extended panels whenever possible. They provide the insight needed to create precision strategies for mood, sleep, and energy restoration.

What happens next at BalanSoul
  • Start with a free 15-minute discovery call— We’ll listen to your concerns, answer questions, and outline which tests or packages are most relevant. (Booking link below.)
  • Full initial consultation (90 minutes)— A deeper review of your health history, symptoms, and any past results. This lets us interpret your lab findings in the context of your whole wellbeing.
  • Targeted, personalised plan— Once results are in, we’ll design a plan that may include circadian-sleep hygiene, nutrition, stress recovery, adaptogens, and nutrient therapy. If needed, we collaborate with your GP or specialist for comprehensive care.

💡 There’s no obligation — just clarity about your next best step.

💬 “Your daily stress rhythm shapes your mood, energy, and sleep. This test shows you where you stand — and how to rebalance. Ready to take the first step? Book your free 15-minute discovery call today.”

Book your Appointment

Use the calendar below to select your preferred appointment date and time. Available dates are highlighted in blue.

15-MINUTE FREE DISCOVERY CALL Confused about testing? Talk to me first.

I’m the practitioner behind BalanSoul. In 15 minutes we’ll map your best next step what to test (and what not to), likely costs/timing, and how it links to your mental health goals.

Free, no obligation, no strings – no surprises.